Coactiv+™ Technology & Biofilms

Plaque and tartar form in your pet’s mouth because of bacteria. Certain bacteria have the ability to produce biofilm – a glue-like substance that functions as a protective dome, under which bacteria can rapidly multiply. When plaque biofilm is not removed from the teeth every 12-36 hours it will begin to bind with minerals in the saliva and calcify into tartar. Excess tartar can work its way up into the gum line causing gingivitis and periodontal disease. Bad breath is usually the first telltale sign that a pet has underlying oral health issues.

Bluestem’s coactiv+™ technology is a safe and effective approach to affecting plaque and tartar by softening and breaking down the biofilm structure without any harsh chemicals or antimicrobials. We’ve invested in clinical trials that show our bluestem™ water additive is effective in reducing tartar, without any brushing, and is safe for daily use at well over the recommended dose.

Here’s how it actually works:

Coactiv+ diagram legend

Without oral care, plaque biofilm and calcified tartar form

A pet’s saliva along with food debris on their teeth contain magnesium, calcium and iron – the food source for bacteria. As bacteria are attracted to these minerals, they attach to the tooth’s surface and produce plaque biofilm, which quickly progresses to calcified tartar.

Step 1: Coactiv+™ binds minerals

When a bluestem™ product enters the mouth, these minerals immediately attach to the binding agents in our coactiv+™ formula, making them unavailable to the bacteria.

Step 2: Without minerals available bacteria begin to die

Coactiv+™ then removes the minerals from the mouth, leaving bacteria without a food source to live on. Bacteria biofilms become weak and eventually the layers of plaque buildup start to fall away.

Step 3: Weakened tartar softens and begins to break off

Hard, calcified tartar begins to soften. It can then easily flake and break off enabling chews, wipes, toothpastes and dental diets to be much more effective.

What are biofilms and how does coactiv+ work?

Watch this video to get an in-depth analysis into what biofilms are and how our coactiv+ technology helps.

In Vivo Study: Dramatic Tartar Softening

In a small-scale in vivo study, 3 dogs were administered our bluestem™ Oral Spray at 2 pumps per side, 2 times per day. Other than their normal food diet, no other products were provided to the dogs. Here is the result for one of the trial dogs:

bluestem in vivo study - day 0

Day 0

Breed: Basset Hound
Age / Sex: 10 year old male
Condition: Severe case of calcified tartar

bluestem in vivo study - day 28

Day 28

Biofilm has been weakened, and there is a noticeable difference in the colour of the tartar.

bluestem in vivo study - day 35

Day 35

Large chunks of calcified tartar have broken off after receiving a hard bone regularly provided to the dog prior to the study.

About Stemcare Animal Health & Kane Biotech

Kane Biotech was formed in 2001 as a discovery and development company focused on novel technologies that prevent and disperse bacterial biofilms. Since then, Kane has worked to translate these discoveries into innovative solutions for human and animal health, as well as industrial applications.

Our bluestem™ oral care water additive was our first product for animal health using our patent-pending coactiv+™ technology. With significant success in the results of our clinical efficacy and safety studies, we decided to launch a full line up of products in the pet oral care category to better support pet parents and the unique personalities of their pets, because the best oral care product is the one you’ll actually use.

For more information on Kane Biotech and biofilms, go to

Kane BioTech researcher